Recovery of Proteins Affected by Mobile Phase Trifluoroacetic Acid Concentration in Reversed-Phase Chromatography

Quantitation of urinary uric acid by reversed-phase liquid chromatography.

The proposed isocratic liquid-chromatographic method for uric acid has good performance characteristics. Results by this procedure and by an enzymic method for uric acid correlated well (r = 0.986). Our method is inexpensive, rapid, and considerably simpler than other techniques for determining uric acid in urine.

Normal-phase liquid chromatography of plant hormones using reversed cholic acid micelles as the mobile phase.

When several plant hormones with similar structures are present in a sample, conventional analytical methods are not sufficiently selective to effectively differentiate them. However, the excellent selectivity of micellar liquid chromatography allows similarly structured compounds to be easily differentiated. We used reversed cholic acid micelles dissolved in tetrahydrofuran as the mobile phase...

Reversed Phase Chromatography

EVALUATION OF THE USE OF ANIONIC/ NONIONIC MIXED MICELLES IN REVERSED PHASE LIQUID CHROMATOGRAPHY OF CHLOROPHENOLS

The use of aqueous mixed micellar system consisting of sodium dodecyl sulfate (SDS) and polyoxyethylene (23) dodecanol (Brij-35) as the mobile phase in reversed-phase liquid chromatography was studied. A group of chlorophenols was used as the test mixture. Adding organic modifier to the system showed that the use of low concentrations of organic additives improves efficiency in SDS/Brij-35 mixe...

Recovery of picloram and 2,4-dichlorophenoxyacetic acid from aqueous samples by reversed-phase solid-phase extraction.

Extensive preparation of samples before chromatographic analysis is usually the most time-consuming process in the determination of many organic compounds in environmental matrices. In the past, removal of some organic8 from aqueous ,mlution was commonly done by liquid/liquid extraction. However, the introduction of stable, covalently bonded reversed-phase sorbents now allows efficient removal ...